Functional analysis of Hyaloperonospora arabidopsidis RXLR effectors.
Identifieur interne : 001084 ( Main/Exploration ); précédent : 001083; suivant : 001085Functional analysis of Hyaloperonospora arabidopsidis RXLR effectors.
Auteurs : Michiel J C. Pel [Pays-Bas] ; Paul C A. Wintermans [Pays-Bas] ; Adriana Cabral [Pays-Bas] ; Bjorn J M. Robroek [Pays-Bas] ; Michael F. Seidl [Pays-Bas] ; Jaqueline Bautor [Allemagne] ; Jane E. Parker [Allemagne] ; Guido Van Den Ackerveken [Pays-Bas] ; Corné M J. Pieterse [Pays-Bas]Source :
- PloS one [ 1932-6203 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Arabidopsis, Immunité des plantes, Protéines végétales.
- microbiologie : Maladies des plantes.
- pathogénicité : Oomycetes.
- Interactions hôte-pathogène, Virulence.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Arabidopsis, Plant Immunity.
- microbiology : Plant Diseases.
- pathogenicity : Oomycetes.
- Host-Pathogen Interactions, Virulence.
Abstract
The biotrophic plant pathogen Hyaloperonospora arabidopsidis produces a set of putative effector proteins that contain the conserved RXLR motif. For most of these RXLR proteins the role during infection is unknown. Thirteen RXLR proteins from H. arabidopsidis strain Waco9 were analyzed for sequence similarities and tested for a role in virulence. The thirteen RXLR proteins displayed conserved N-termini and this N-terminal conservation was also found in the 134 predicted RXLR genes from the genome of H. arabidopsidis strain Emoy2. To investigate the effects of single RXLR effector proteins on plant defense responses, thirteen H. arabidopsidis Waco9 RXLR genes were expressed in Arabidopsis thaliana. Subsequently, these plants were screened for altered susceptibility to the oomycetes H. arabidopsidis and Phytophthora capsici, and the bacterial pathogen Pseudomonas syringae. Additionally, the effect of the RXLR proteins on flg22-triggered basal immune responses was assessed. Multifactorial analysis of results collated from all experiments revealed that, except for RXLR20, all RXLR effector proteins tested affected plant immunity. For RXLR9 this was confirmed using a P. syringae ΔCEL-mediated effector delivery system. Together, the results show that many H. arabidopsidis RXLR effectors have small effects on the plant immune response, suggesting that suppression of host immunity by this biotrophic pathogen is likely to be caused by the combined actions of effectors.
DOI: 10.1371/journal.pone.0110624
PubMed: 25375163
PubMed Central: PMC4222755
Affiliations:
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Pel</name><affiliation wicri:level="4"><nlm:affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Wintermans, Paul C A" sort="Wintermans, Paul C A" uniqKey="Wintermans P" first="Paul C A" last="Wintermans">Paul C A. Wintermans</name><affiliation wicri:level="4"><nlm:affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Cabral, Adriana" sort="Cabral, Adriana" uniqKey="Cabral A" first="Adriana" last="Cabral">Adriana Cabral</name><affiliation wicri:level="4"><nlm:affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Robroek, Bjorn J M" sort="Robroek, Bjorn J M" uniqKey="Robroek B" first="Bjorn J M" last="Robroek">Bjorn J M. Robroek</name><affiliation wicri:level="4"><nlm:affiliation>Ecology and Biodiversity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Ecology and Biodiversity, Department of Biology, Faculty of Science, Utrecht University, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Seidl, Michael F" sort="Seidl, Michael F" uniqKey="Seidl M" first="Michael F" last="Seidl">Michael F. Seidl</name><affiliation wicri:level="4"><nlm:affiliation>Centre for BioSystems Genomics, Wageningen, The Netherlands; Theoretical Biology and Bioinformatics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Centre for BioSystems Genomics, Wageningen, The Netherlands; Theoretical Biology and Bioinformatics, Department of Biology, Faculty of Science, Utrecht University, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Bautor, Jaqueline" sort="Bautor, Jaqueline" uniqKey="Bautor J" first="Jaqueline" last="Bautor">Jaqueline Bautor</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Parker, Jane E" sort="Parker, Jane E" uniqKey="Parker J" first="Jane E" last="Parker">Jane E. Parker</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Van Den Ackerveken, Guido" sort="Van Den Ackerveken, Guido" uniqKey="Van Den Ackerveken G" first="Guido" last="Van Den Ackerveken">Guido Van Den Ackerveken</name><affiliation wicri:level="4"><nlm:affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Pieterse, Corne M J" sort="Pieterse, Corne M J" uniqKey="Pieterse C" first="Corné M J" last="Pieterse">Corné M J. Pieterse</name><affiliation wicri:level="4"><nlm:affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Oomycetes (pathogenicity)</term><term>Plant Diseases (microbiology)</term><term>Plant Immunity (genetics)</term><term>Plant Proteins (genetics)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (génétique)</term><term>Immunité des plantes (génétique)</term><term>Interactions hôte-pathogène (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Oomycetes (pathogénicité)</term><term>Protéines végétales (génétique)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Plant Immunity</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Immunité des plantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Oomycetes</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Oomycetes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Host-Pathogen Interactions</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Interactions hôte-pathogène</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The biotrophic plant pathogen Hyaloperonospora arabidopsidis produces a set of putative effector proteins that contain the conserved RXLR motif. For most of these RXLR proteins the role during infection is unknown. Thirteen RXLR proteins from H. arabidopsidis strain Waco9 were analyzed for sequence similarities and tested for a role in virulence. The thirteen RXLR proteins displayed conserved N-termini and this N-terminal conservation was also found in the 134 predicted RXLR genes from the genome of H. arabidopsidis strain Emoy2. To investigate the effects of single RXLR effector proteins on plant defense responses, thirteen H. arabidopsidis Waco9 RXLR genes were expressed in Arabidopsis thaliana. Subsequently, these plants were screened for altered susceptibility to the oomycetes H. arabidopsidis and Phytophthora capsici, and the bacterial pathogen Pseudomonas syringae. Additionally, the effect of the RXLR proteins on flg22-triggered basal immune responses was assessed. Multifactorial analysis of results collated from all experiments revealed that, except for RXLR20, all RXLR effector proteins tested affected plant immunity. For RXLR9 this was confirmed using a P. syringae ΔCEL-mediated effector delivery system. Together, the results show that many H. arabidopsidis RXLR effectors have small effects on the plant immune response, suggesting that suppression of host immunity by this biotrophic pathogen is likely to be caused by the combined actions of effectors. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25375163</PMID><DateCompleted><Year>2015</Year><Month>07</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>11</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Functional analysis of Hyaloperonospora arabidopsidis RXLR effectors.</ArticleTitle><Pagination><MedlinePgn>e110624</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0110624</ELocationID><Abstract><AbstractText>The biotrophic plant pathogen Hyaloperonospora arabidopsidis produces a set of putative effector proteins that contain the conserved RXLR motif. For most of these RXLR proteins the role during infection is unknown. Thirteen RXLR proteins from H. arabidopsidis strain Waco9 were analyzed for sequence similarities and tested for a role in virulence. The thirteen RXLR proteins displayed conserved N-termini and this N-terminal conservation was also found in the 134 predicted RXLR genes from the genome of H. arabidopsidis strain Emoy2. To investigate the effects of single RXLR effector proteins on plant defense responses, thirteen H. arabidopsidis Waco9 RXLR genes were expressed in Arabidopsis thaliana. Subsequently, these plants were screened for altered susceptibility to the oomycetes H. arabidopsidis and Phytophthora capsici, and the bacterial pathogen Pseudomonas syringae. Additionally, the effect of the RXLR proteins on flg22-triggered basal immune responses was assessed. Multifactorial analysis of results collated from all experiments revealed that, except for RXLR20, all RXLR effector proteins tested affected plant immunity. For RXLR9 this was confirmed using a P. syringae ΔCEL-mediated effector delivery system. Together, the results show that many H. arabidopsidis RXLR effectors have small effects on the plant immune response, suggesting that suppression of host immunity by this biotrophic pathogen is likely to be caused by the combined actions of effectors. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pel</LastName><ForeName>Michiel J C</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wintermans</LastName><ForeName>Paul C A</ForeName><Initials>PC</Initials><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cabral</LastName><ForeName>Adriana</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Robroek</LastName><ForeName>Bjorn J M</ForeName><Initials>BJ</Initials><AffiliationInfo><Affiliation>Ecology and Biodiversity, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seidl</LastName><ForeName>Michael F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Centre for BioSystems Genomics, Wageningen, The Netherlands; Theoretical Biology and Bioinformatics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bautor</LastName><ForeName>Jaqueline</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parker</LastName><ForeName>Jane E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van den Ackerveken</LastName><ForeName>Guido</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pieterse</LastName><ForeName>Corné M J</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; Centre for BioSystems Genomics, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>11</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant 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Wintermans</name></country><country name="Allemagne"><noRegion><name sortKey="Bautor, Jaqueline" sort="Bautor, Jaqueline" uniqKey="Bautor J" first="Jaqueline" last="Bautor">Jaqueline Bautor</name></noRegion><name sortKey="Parker, Jane E" sort="Parker, Jane E" uniqKey="Parker J" first="Jane E" last="Parker">Jane E. Parker</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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